Size Exclusion Chromatography (SEC) is a critical technique in biopharmaceutical research and development, demanding high resolution, efficiency, and reliability. The SRT-10 SEC column series is engineered to meet these demands, utilizing proprietary surface technologies to deliver exceptional performance in biomolecule separation and analysis. These columns are designed for scientists and researchers who require robust and reproducible separation of proteins, antibodies, nucleic acids, polymers, and other biological macromolecules.
Advanced SEC Surface Chemistry: The SRT-10 Advantage
The foundation of SRT-10 and SRT-10C SEC phases lies in their innovative surface chemistry. These columns are constructed with uniform, hydrophilic, and neutral nanometer-thick films chemically bonded to high-purity, mechanically stabilized 10 µm silica particles. This meticulous surface engineering ensures minimal non-specific interactions and outstanding batch-to-batch reproducibility, crucial for consistent and dependable results.
The unique bonding chemistry maximizes bonding density, contributing to the high stability and negligible non-specific binding characteristic of SRT-10 and SRT-10C phases. This is visually represented in the surface monolayer comparison:
As illustrated, SRT-10 (and SRT-10C) employs a “stand-up” monolayer, contrasting with the “lay-down” monolayer of Zenix columns. This difference is key to their specialized applications:
- SRT-10: Ideal for non-hydrophobic samples, including a wide array of proteins such as Monoclonal Antibodies (mAbs) and various other non-hydrophobic proteins.
- SRT-10C: Designed for hydrophobic samples like Antibody-Drug Conjugates (ADCs), derivatized mAbs, pegylated proteins, membrane proteins, and “super sticky” proteins that pose challenges in traditional SEC.
This tailored surface chemistry ensures optimal performance and minimizes sample loss or distortion due to unwanted interactions.
Experience Fast, High-Resolution Purification with SRT-10 on FPLC Systems
In fast protein liquid chromatography (FPLC), speed and resolution are paramount. SRT-10 SEC columns excel in delivering both, significantly reducing run times without compromising separation quality. The large pore volume of SRT-10 packings contributes to high separation resolution, allowing for the effective purification and analysis of complex biomolecules.
Consider the comparative data highlighting the efficiency of SRT-10 SEC-300 columns in FPLC:
| Product | Sepax SRT/SRT-C SEC-300 21.2x300mm, 5µm | Sepax SRT-10/10C SEC-300 21.2x300mm, 10µm | GE Superdex 200 16x600mm, 34µm |
|---|---|---|---|
| Product Code | 215300-21230/ 235300-21230 | 225300-21230/ 239300-21230 | 28-9893-35 |
| Column Volume | 105 mL | 105 mL | 120 mL |
| Flow Rate | 7-10 mL/min | 7-10 mL/min | 0.75-1 mL/min |
| Run Time | > 2 Hour | ||
| Backpressure | 55-70 bar | 15-27 bar (well below AKTA FPLC pressure limit) | Superdex max 3 bar tolerance |
| Max Loading | 5 mL | ~42mg (BSA) / 5ml | 5ml |
| Characteristics | Fast High Res Purification | Fast Purification |
SRT-10 columns can drastically reduce your purification time, cutting run times from 2 hours down to approximately 20 minutes. This represents an impressive 85% time saving while maintaining or even enhancing resolution. The lower backpressure of SRT-10 columns, especially the 10 µm particle size, is also advantageous, operating well within the pressure limits of common FPLC systems like AKTA FPLC, ensuring system longevity and robustness.
This efficiency gain translates directly to increased throughput and faster results in your research or bioprocessing workflows.
SRT-10 Technical Specifications: Pore Size and Performance
The SRT-10 SEC series is available in a range of pore sizes to accommodate different molecular weight ranges, ensuring optimal separation for your target molecules. The narrowly dispersed, spherical silica particles are manufactured with precise pore sizes, including 100 Å, 300 Å, 500 Å, and 2000 Å.
Here’s a detailed look at the technical specifications of SRT-10 and SRT-10C columns:
| Phase | SRT-10/10C SEC-100 | SRT-10/10C SEC-300 | SRT-10/10C SEC-500 | SRT-10/10C SEC-1000 |
|---|---|---|---|---|
| Material | Neutral, hydrophilic film bonded silica | |||
| Particle size | 10 µm | 10 µm | 10 µm | 10 µm |
| Pore size (Å) | ~ 100 | ~ 300 | ~ 500 | ~ 1000 |
| Protein MW range (native) | 100 – 30,000 | 5,000 – 1,250,000 | 15,000 – 5,000,000 | 50,000 – 7,500,000 |
| pH stability | 2.5 – 7.5 (up to pH 8.5 can be tolerated temporarily*) | |||
| Backpressure (7.8×300 mm) | ~ 300 psi (flow rate at 1.0 mL/min) | |||
| Maximum backpressure (psi) | ~ 3,000 | |||
| Salt concentration range | 20 mM – 500 mM | |||
| Maximum temperature (°C) | ~4 – 40°C | |||
| Mobile phase compatibility | Aqueous and organic |
This range of pore sizes allows researchers to select the optimal SRT-10 column for their specific separation needs, ensuring high-resolution separation across a broad spectrum of biomolecule sizes.
Sample Loading Recommendations for Optimal SRT-10 Performance
Proper sample loading is crucial for achieving optimal separation and column longevity. SRT-10 columns offer flexible loading capacities depending on the column dimensions.
| ID | 4.6×300 mm | 7.8×300 mm | 10×300mm | 21.2×300mm | 30×300mm | 50×300mm |
|---|---|---|---|---|---|---|
| Column Volume | 5 mL | 14 mL | 24 mL | 105 mL | 212 mL | 490 mL |
| V-injection | 5-100 µL | 15-250 µL | 25-940 µL | 0.1-4.2 mL | 0.2-8.5 mL | 0.5-20 mL |
| Column Loading Guideline (BSA) | ||||||
| Standard Flow rate (Maximum) | 0.35 mL/min | 1.0 mL/min | 1.65 mL/min (2.0 mL/min) | 7.5 mL/min (10mL/min) | 15 ml/min (25 ml/min) | 41 ml/min (60 ml/min) |
| Typical Run Time | ||||||
| Back pressure |
These guidelines provide a starting point for optimizing your separation conditions, and further adjustments may be necessary based on your specific sample and experimental setup.
Key Advantages of SRT-10 SEC Columns
SRT-10 and SRT-10C SEC columns offer a compelling combination of features and benefits:
- 10 µm Particle Size: Provides an excellent balance of resolution and backpressure.
- Versatile Pore Sizes (100, 300, 500, 2000 Å): Optimized for a wide range of biomolecule sizes.
- Broad pH Stability (2-8.5): Compatible with various mobile phase conditions.
- High Capacity and Resolution: Delivers sharp, well-resolved peaks.
- High Stability: Robust performance across low and high salt concentrations.
- Exceptional Lot-to-Lot Reproducibility: Ensures consistent results over time.
- High Protein Recovery: Maintains the biological activity of purified proteins.
- Negligible Non-Specific Interactions: Minimizes sample loss and distortion.
- Ideal for Biological Molecules: Effective separation of proteins, nucleic acids, oligonucleotides, peptides, and viruses.
- Suitable for Natural and Synthetic Polymers: Separation of polysaccharides, water-soluble synthetic polymers, and nanomaterials.
Broad Applications in Biomolecule Separation
SRT-10 and SRT-10C columns are versatile tools applicable across a wide range of research areas. They are particularly well-suited for:
- Protein Purification and Characterization: From monoclonal antibodies to complex protein mixtures.
- Antibody Aggregation Analysis: Ensuring the quality and stability of antibody therapeutics.
- Polymer Analysis: Size distribution analysis of natural and synthetic polymers.
- Nanoparticle Separation: Characterization of nanomaterials and drug delivery systems.
Selected Literature References:
Numerous publications highlight the successful application of SRT-10 and SRT-10C columns in cutting-edge research. Examples include:
- Characterization of a Novel Bispecific Antibody That Activates T Cells In Vitro and Slows Tumor Growth In Vivo (Janssen, using SRT-10C SEC-300) doi.org/10.1089/mab.2019.0035
- A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells (Genentech, using SRT-10 SEC-300) doi.org/10.1038/s41589-018-0035-2
- PROMIS: Global Analysis of PROtein-Metabolite Interactions (Max-Planck Institute, using SRT-10 SEC-300) doi.org/10.1074/jbc.ra118.003351
These are just a few examples demonstrating the broad utility and impact of SRT-10 SEC columns in advancing scientific discovery.
Conclusion: Elevate Your Separations with SRT-10
SRT-10 SEC columns represent a significant advancement in size exclusion chromatography. Their innovative surface chemistry, combined with a range of pore sizes and robust performance characteristics, makes them an ideal choice for researchers seeking high-resolution, fast, and reliable separation of biomolecules. Whether you are working with proteins, antibodies, polymers, or nanoparticles, SRT-10 columns provide the tools you need to achieve superior separation and accelerate your research.
Explore the SRT-10 SEC column series today and experience the difference in your biomolecule separations.
